Cell-Based Assays for Studying Cell Function, Proliferation and Toxicity

  • Cell viability assay: e.g., CellTiter-Glo® (Promega) ATP assay for detection of viable cells
  • Cytotoxicity assays: e.g., CytoTox-ONETM (Promega) to estimate number of nonviable cells by measuring LDH release
  • Apoptosis assays: e.g., Caspase-Glo® 3/7 Assay (Promega)
  • Impedance-based in-vitro real time monitoring of adherent cell growth, viability and toxicity via xCELLigence® (Agilent) System
  • Preparation (magnetic beads) and differentiation of specific cell types
  • Flow cytometry to analyse surface markers or cell subpopulations
  • Cytospin to perform thin-layer preparations from fluids through a cytocentrifugation process followed e.g., by differential cell counts
  • Ago-2 Loading Assay: co-immunoprecipitation of Ago-2 protein with bound siRNA or miRNA and quantification by stem loop PCR