We offer bioinformatics analyses both as a standalone service and to support lead identification programs and other biological studies.

Lead identification

An upstream bioinformatics assessment is performed for an in-silico sequence pre-selection that includes:

• Proprietary algorithm for selection of specific oligonucleotide (avoiding off-targets)  especially for siRNAs and ASOs
• Considering species cross-reactivity  
• Oligonucleotide design (structure and chemistry)  
• For siRNAs, gapmer ASOs (established pipelines) 
• Custom oligonucleotide design projects (DNAzymes, miRNA-like oligos)
• Allele-(SNPs) and fusion gene specific targeting
• Design of reporter plasmids
• Design of scrambled control oligonucleotides 

Lead optimization 

• Consultancy to improve activity, stability and specificity, oligo structure and sequence as well as chemical modification

Off-target analysis

• Detailed listing and characterization of predicted off-targets
• RNA-Seq analysis
• Propose cell lines for off-target analysis
• Design of
  
  • Reporter plasmids for off-target analysis
  • Reporter for siRNA sense and antisense strand activity
  • Reporter for seed mediated miRNA-like activity 
     

Support for biological services 

• Primer design for SANGER sequencing and 5’-RACE
• Processing of 5‘-RACE data  
• Automated data processing (e.g., Meso Scale Discovery)
• Processing and analysis of sequencing results