Lipid nano particle (LNP) formulation 

• In our organic synthesis lab synthesized lipids are assembled to yield LNPs
  • Our proprietary lipids for nanoparticle formulation
  • Lipids received from collaborating partners can also be integrated
• Capable of loading varied cargos: RNA, DNA, sgRNA, etc.
• Ready to use formulations for in-vitro studies as well as for studies in rodents and higher species
  
  • Size
  • Polydispersity
  • Zeta potential
  • UV and fluorescence-based quantification and entrapment evaluation

Characterization

• Analyses of lipid stability in lipid nanoparticles (LNPs)
• LC-MS based quantification of lipids in tissues upon in vivo LNP administration
• In vitro and in vivo functional analysis of oligonucleotides by monitoring mRNA and protein content as well as observation of phenotypic changes
• In vivo test system to monitor cell type-specific oligonucleotide drug delivery to multiple tissues 
  • Established LNPs known to deliver preferentially to certain cell types like Stellate and Kupffer cells and others, based on our proprietary platform of LNPs
  • Established siRNAs targeting cell-type specific marker genes to monitor successful delivery to certain cell types in liver and lung