Capabilities

• State-of-the-art facilities
  • Safety level 1 facility
  • Safety level 2 laboratory
• Custom-tailored preclinical services
• Lead identification and characterization
• Off-target analysis
• In vivo test system to monitor cell type-specific oligonucleotide drug delivery to multiple tissues
• We have established LNPs known to deliver to preferentially to certain cell types
• We use established siRNAs targeting cell-type specific markers to monitor successful delivery to these cell types
• Analysis in primary hepatocytes from various species
• Flow cytometry
• 5’-RACE to analyse oligonucleotide-mediated, sequence-specific mRNA cut 

Functional Analyses

• 5'-RACE to identify the oligonucleotide-mediated cleavage site in mRNA
• AGO2-Loading Assay: Co-immunoprecipitation and stemloop-PCR for relative quantification of the siRNA strand or miRNA strand loaded in AGO2

Quantification and analysis of cellular mRNA

• see mRNA Quantification

Quantification and analysis of protein 

• see Protein Analysis