High Throughput Screening
- We have more than 200 different cell lines on stock
- Typically, screening of several hundred oligonucleotides in dual dose and further characterization of a smaller selection in dose response curves
- Individual target-specific assay development
- Analysis of target expression in cell lysates by QuantiGene™ singleplex assay (bDNA) or qPCR
- Oligo screening of endogenously expressed targets in a cell line (alternatively screen by psiCHECK™ - 2 (Promega) reporter assay)
- Determination of IC50, IC80 and max. Knockdown
- For e.g., viral targets: oligo screen by psiCHECK™-2 Reporter assay
- Dual-Glo®-Assay (Promega) used for readout
- Screening in primary hepatocytes or iPSC (induced pluripotent stem cells) derived cell types by transfection, receptor-mediated or direct uptake
