Biology & Pharmacology 

Molecular and Cellular Biology and in vivo Capabilities

State-of-the-art facilities

  • S1 facility
  • Safety level 2 cell culture lab

Safety and toxicology analyses

  • PBMC assays to measure oligonucleotide-induced cytokine response
  • Clinical chemistry; analysis of e.g., ALB, ALKPH, ALAT, ASAT, BILI, Ca, CHOL, CREAT, GGT, GKUC, Mg, P, Protein, TRIG or Carbamide from biological fluids like serum, plasma or Urine (COBAS Integra)
  • Differential cell counts from fluids after cytospin (Shandon)

Ligand-receptor interaction and uptake studies/histology

  • Confocal LSM710 (Zeiss) for analysis with up to four channels in parallel
  • Fluorescence microscopy (ApoTome, Zeiss)
  • Histological sections from frozen and paraffin-embedded tissues

Analysis of mRNA up- and downregulation

  • Quantigene® branched DNA assay
  • Quantitative RT-PCR
  • In situ hybridization

Cell-based assays for cell function, proliferation and toxicity

  • In vitro monitoring of cell number and cell viability via xCelligence System
  • Preparation (magnetic beads) and differentiation of specific cell types
  • Standard cytotox and apoptosis assays

Custom-tailored preclinical Services

CRISPR/Cas-related technologies

Lead identification and characterization

In vivo models to monitor cell type-specific oligonucleotide drug delivery to multiple tissues

Analysis in primary hepatocytes from various species

Protein analysis

  • Quantification of multiple proteins from the same sample, as e.g. cytokine panels, byLuminex® Multiplex Reader or Meso Quickplex
  • Classical ELISA and MSD-ELISA

Flow cytometry

  • BD-LSR II for detection of up to 12 different fluorescent labels
  • HTS device for 96-well plates