Biology & Pharmacology 

Molecular and Cellular Biology and in vivo Capabilities

State-of-the-art facilities

  • Safety level 1 facility
  • Safety level 2 laboratory

Safety and toxicology analyses

  • PBMC assays to measure oligonucleotide-induced cytokine response
  • Clinical chemistry; analysis of 36 different parameters from biological fluids like serum, plasma or urine (COBAS Integra)
  • Differential cell counts from fluids after cytospin

Ligand-receptor interaction and uptake studies/histology

  • Confocal microscopy (Zeiss) for analysis with up to four channels in parallel
  • Fluorescence microscopy (Zeiss)
  • Histological sections from paraffin-embedded tissues

Nucleic acid analysis and quantification of mRNA

  • Quantigene® branched DNA assay
  • Quantitative RT-PCR (QuantStudio)
  • In situ hybridization
  • Tape station electrophoresis

Cell-based assays for cell function, proliferation and toxicity

  • In vitro monitoring of cell number and cell viability via xCelligence System
  • Preparation (magnetic beads) and differentiation of specific cell types
  • Standard cytotox and apoptosis assays

Custom-tailored preclinical services

CRISPR/Cas-related technologies

  • Activity testing of sgRNAs
  • Determination of genome editing efficiency by T7 endonuclease I + TIDE assay

Lead identification and characterization

In vivo models to monitor cell type-specific oligonucleotide drug delivery to multiple tissues

Analysis in primary hepatocytes from various species

Protein analysis

  • Quantification of multiple proteins from the same sample, as e.g. cytokine panels, by Luminex® Multiplex Reader or Meso Quickplex
  • Classical ELISA and MSD-ELISA
  • WES Simple Western

Flow cytometry