Bioanalytics of Oligonucleotides and mRNA 

Axolabs has a unique and proprietary assay system for the sensitive detection of oligonucleotides from biological matrices for the analysis and characterization of DMPK and ADME properties of oligonucleotide-based therapeutics

  • Sensitive detection of single- and double-stranded oligonucleotides
  • Extraction-free and robust procedure with the option of parallel quantification of oligonucleotide and target mRNA from a single sample
  • Assay compatible with conjugates and different oligonucleotide chemistries
  • Simultaneous detection of metabolites
  • Downstream mass spectrometric identification of oligonucleotide metabolites
  • Assay established in regulated environment (GLP/GCP)
  • Detection of 5’-phosphorylation of siRNAs as a marker for cytoplasmic delivery and a unique tool to determine PK/PD correlation

Quantitative detection of mRNA therapeutics including PK/TK of mRNA therapeutics from various biological matrices by:

  • qRT-PCR using SYBR Green or TaqMan
  • Quantigene® branched DNA assay
    • Sensitive and extraction-free (no mRNA extraction necessary)
    • Parallel quantitative detection of oligonucleotide and target mRNA from a single sample
    • Assay established in regulated environment (GLP/GCP)